Análisis microscópico de la adherencia in vitro de Candida albicans en resina acrílica utilizada para bases de dentaduras procesadas con tres diferentes técnicas

Abstract

Antecedents: Prosthetic stomatitis is associated to the adherent and colonization of Candida albicans to denture bases and other cofactors such as acidic pH, high carbohidrate diet, systemic diseases as well as pharmacological treatments. Objective: To analyze Candida albicans adherence in vitro on different samples of polymetacrilate acrylic resin used for denture base, processed with three different techniques using scanning electron microscopy (SEM). Study design: 18 different samples of acrylic resin were made using three different techniques. Candida albicans was inoculated in vitro for 24 and 48 hours. These samples were observed under the microscope, getting pictures of them at 600x, 1,500x and 6,000x magnification. Results: The resin elaborated with thermopolimerization injection (TPI) presents pores of 1-2 microns in diameter. At 24 hours after inoculation blastoconidios can be observed, as well as the formation of pseudomycelium while at 48 there are numerous cells in gemmation. In auto and termopolimerization injection (ATPI) there are pores measuring .5-5 microns in diameter as well as cracks with 100 microns in length. At 24 hours the blastoconidios have irregular shapes while at 48 hours there are pseudomycelium, gemmation and cells present irregularities. In microwave polimerization (MP) there are pores with 1-5 microns in diameter. At 24 hours there are pseudomycelium anode cells in gemmation, while at 48 hours the pseudomycelium formation as well as gemmation is increased. Conclusions: Acrylic resin elaborated with the thermopolimerization injection technique presents a surface with less defects of the three studied techniques and there is no significant difference in the number of adhered cells at 24 and 48 hours after inoculation.