Influence on the Number of Shock Waves and Pressure in Pulp Cells and Osteoblasts
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Abstract
Introduction: Biomedical applications of shock waves have been developed to treat various conditions. Objective: To evaluate the effectiveness of extracorporeal shock waves according to the pressure and number of waves applied to stimulate the proliferation of cultured human odontoblasts and dental pulp stem cells. Material and methods: Prospective, comparative, in vitro experimental study. Approximately 6×105 cells/ml of human bone and dental pulp cells were inoculated, and different numbers of shock waves were applied to randomly form 8 groups per cell type. Results: The number of shock waves and their positive pulse pressure influence cell viability. By applying 400 shock waves at a pressure of approximately 22 MPa to osteoblasts, a 50% increase in cell viability was obtained at 48 hours. Conclusions: The use of low pressure and a high number of shock waves increases cellular activity.
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References
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